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KMID : 1101420190510020245
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Korean Journal of Clinical Laboratory Science
2019 Volume.51 No. 2 p.245 ~ p.251
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Effects of 2-methoxy-1,4-naphthoquinone (MQ) on MCP-1 Induced THP-1 Migration
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Kim Si-Hyun
Park Bo-Bin
Hong Sung-Eun
Yu Sung-Ryul
Lee Jang-Ho
Kim Sa-Hyun
Lee Pyeong-Jae
Cho Eun-Kyung
Moon Cheol
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Abstract
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This study examined the effects of 2-methoxy-1,4-naphthoquinone (MQ) on the monocyte chemoattractant protein-1 (MCP-1)-induced migration of monocytes, which is an important phenomenon for the body defense and immune response. MQ is a major component extracted from Impatiens balsamina leaves, which have been used for many years in Asian medicine for the treatment of a range of diseases and pain. The cytotoxicity of MQ began to appear at a concentration of 10 μM, and approximately 50% cytotoxicity was confirmed at 100 μM. The MCP-1 induced migration of the THP-1 monocyte cell line increased after MQ treatment in a dose dependent manner and the largest increase was observed at 0.1 μM. The level of cAMP expression decreased after a treatment with 0.1 μM MQ. The phosphorylation of extracellular signal-regulated kinases 1/2 (Erk1/2), a key signaling protein involved in the signaling pathway of C-C motif chemokine receptor 2 (CCR2), a receptor for MCP-1, was increased by the simultaneous treatment of 0.1 μM MQ. These results show that MQ increases the MCP-1-induced migration of THP-1, decreases the level of cAMP expression, and increases the level of Erk1/2 phosphorylation.
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KEYWORD
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2-methoxy-1,4-naphthoquinone, Migration, Monocyte, Monocyte chemoattractant protein 1
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